I assume you want to block port 25 so email apps (Thunderbird, Outlook, Apple mail, ect) cannot use it to send e-mail because it's an insecure connection?
However port 25 is also used by a sever for talking to other (mail) servers. So if you want send email to an Gmail email address (for example) your server cant connect to to the Gmail servers to send the email. So if you block 25 no email can be send at all.